Structural and Compositional SOPs

ex-vivo nano-Computed Tomography

These protocols are designed for a GE phoenix nanotom® s system and are in the process of being updated for the newer GE phoenix nanotom® m system. If you have a different nano-CT system, you will need to adjust the protocol accordingly.

ex-vivo micro-Computed Tomography

These protocols are designed for a GE eXplore Locus SP ex vivo preclinical specimen scanner. If you have a different micro-CT system, you will need to adjust the protocol accordingly.

Raman Microscope

This SOP describes the use of the Core Center Raman microscope to acquire spectra of fresh or PMMA-embedded bone specimens. The Raman microscope is equipped with a Kaiser Optical System HoloSpec Spectrograph (400-1900 cm-1), HoloSpec Calibration Accessory (HCA), 785-nm Innovative Photonics Solution Laser (IPS Laser Unit), and an Andor Technology Newton back-illuminated CCD camera. The SOP goes over the various safety considerations and the Raman spectral acquisition procedure.

Raman Fiber Probe

This SOP describes the use of the Raman fiber optic probe on fresh soft or hard tissue specimens. The fiber probe is primarily used to collect spectra from specimens with large surface areas, such as rodent skin or calvarial tissues, including human bone sections. The Raman fiber probe is equipped with a Kaiser Optical System HoloSpec Spectrograph (194-1930 cm-1), HoloSpec Calibration Accessory (HCA), 785-nm Invictus Andor Laser, and an Andor Technology back-illuminated CCD camera. The SOP goes over the various safety considerations and the Raman spectral acquisition procedure.