Histology Workflows and SOPs

Workflows for Submitting Requests

Please discuss your project with a Histologist as far in advanced as possible to ensure you will be able to get your specimens processed correctly and within your project deadlines. All workflows have both self-service and/or Core service components.

• Paraffin embedding and sectioning workflow

• Plastic embedding and sectioning workflow

• Cryostat sectioning workflow

SOPs and Protocols

• Cryostat sectioning (non-hazardous)

• Cutting and grinding thick plastic sections using Buehler’s Isomet and EcoMet

• Drying Benzoyl Peroxide

• Embedding Paraffin Samples

• Freezing and Embedding Tissue for Cryostat Sectioning

• Isomet Instructions

• Methyl Methacrylate Solutions and PMMA

• Methyl Methacrylate Processing and Embedding

• Sectioning Paraffin Blocks, Automated

• Sectioning Paraffin Blocks, Manually

• Sectioning Plastic Blocks

• Toluidine Blue / Basic Fuchsin Stain

• TRAP Stain on Thick PMMA Sections of Bone

Booklets

• The "Bone Histomorphometry Booklet" contains all the protocols relative to plastic thin sectioning and histomorphometric analyses used by the P30 Histology Assessment Core. In this booklet, you will find information on preparing and submitting specimens to the Core, embedding the specimens and sectioning, staining the sections, acquiring microscope images of the sections, and histomorphometric analysis of the images.

Contact information for Histology Core

• Karl Jepsen, PhD (kjepsen@med.umich.edu)

• Carol Whitinger (whitinge@med.umich.edu)